SCREENING AND IDENTIFICATION ENDOPHYTIC BACTERIA FROM INDONESIAN BAY LEAVES (Eugenia polyantha WIGHT) WITH ANTIBACTERIA ACTIVITY
SCREENING AND IDENTIFICATION ENDOPHYTIC
BACTERIA FROM INDONESIAN BAY LEAVES (Eugenia polyantha
WIGHT) WITH ANTIBACTERIA ACTIVITY
For many years, herbs already utilized as traditional medicine to cure diseases,
particularly bacterial infections. One of potential herbs which reported has antibacterial activity
is Eugenia polyantha Wight. This herbs is commonly used as spices for food by Indonesian
people. Due to the significant increasing of multidrug resistance bacteria globally, the invention
of new antibiotics is urgently needed. One of alternative ways finding active compounds to solve
the problems is by using microbes inside plants. Unfortunately, no reports about endophytic
bacteria from Indonesian Bay Leaves especially its potential as antibacterial. This research
aimed to find endophytic bacteria isolates from Indonesian Bay Leaves which has antibacterial
activity against Salmonella typhi. First, we isolated the bacteria from fresh E. polyantha leaves
on agar medium then cultivated it using NB medium and continued to antibacterial assay by disc
diffusion. The 16 sRNA of isolate with inhibition ability was sequenced. We got three endophytic
bacteria isolates but only one isolate shown great antibacterial activity. According to the
similarity result by BLAST analysis the isolate had similarity to Bacillus sp, thus, we named the
isolate with B. subtilis strain WL-SM1.
http://repository.uhamka.ac.id/779/1/%28222-227%29V8N10CT.pdf
