Screening of Antibacterial Potency and Molecular Identification of Endophytic Bacteria from Soursop Leaf (Annona muricata L.)

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Screening of Antibacterial Potency and Molecular Identification of Endophytic Bacteria from Soursop Leaf (Annona muricata L.)

http://repository.uhamka.ac.id/818/

Fitri Yuniarti, Fitri

Soursop leaves (Annona muricata L.) is one of the medicinal plants identified as a source of endophytic
bacteria producing secondary metabolites. Several studies have reported that secondary metabolite
compounds extracted from soursop leaves have inhibitory activity against pathogenic bacteria and fungi and
have anticancer activity. This study aims to isolate endophytic bacteria of soursop leaf and identify
molecular isolates producing antibacterial metabolites by PCR method. This study began with endophytic
bacterial isolation of soursop leaves, followed by screening for antibacterial potency using disc diffusion
method and identification of molecular isolates which had the highest antibacterial activity. After isolation,
three isolates were obtained: BW-1LM, BW-2LP, and BW-3LK. The result of antibacterial activity test
showed that BW-1LM isolate had the highest activity against bacterium of Bacillus subtilis and Salmonella
typhi test. Molecular identification was obtained by BW-1LW isolate having 99% similarity level to
Bacillus licheniformis DSM 13 strain. The conclusion is soursop leaves contains endophytic bacteria which
have antibacterial activity against Bacillus subtilis and Salmonella typhi.

http://repository.uhamka.ac.id/818/1/MICH-PhD_2018_29.pdf

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