Utilization of Bromelain Enzyme from Pineapple Peel Waste on Mouthwash Formula Against Streptococcus mutans
Utilization of Bromelain Enzyme from Pineapple Peel Waste on
Mouthwash Formula Against Streptococcus mutans
http://repository.uhamka.ac.id/623/
The aim of this research was to apply bromelain enzyme from rough extract of Ananas
comosus (L.) Merr. peel into the mouthwash preparation and investigate the enzyme activity to
inhibit Streptococcus mutans growth.. The research method, bromelain was extracted by means
of a blended pineapple skin, the extract was filtrated and centrifuged to obtain a supernatant.
Enzyme activity was analyzed by spectrophotometer at 275 nm. Mouthwash formula which
involved different concentration of bromelain (20%, 25%, 30%, 35%, 40%, 50%, and 60% (v/v))
was tested the antimicrobial activity by disc diffusion method. The results showed that the formula
with 35% enzyme was an effective in restricting the growth of Streptococcus mutans, with
